Proteins and hyaluronic acid associated with proteoglycans extracted from bovine nasal cartilage with low-ionic-strength salt solution.
نویسندگان
چکیده
Bankowski, E. & Mitchell, W. M. (1973) Biophys. Chem. 1,73-86 Calatroni, A., Donnelly, P. V. & Di Ferrante, N. (1969) J. Clin. Invest. 48,332-343 Dell’ Orco, R. T., Mertens, J. G. & Kruse, P. F., Jr. (1973) Exp. Cell Res. 77,356-360 Dell’ Orco, R. T. & Nash, J. H. (1973) Proc. SOC. Exp. Biol. Med. 144,621-622 Goldberg, B., Epstein, E. H., Jr. & Sherr, C. J. (1972) Proc. Nut. Acad. Sci. US. 69,3655-3659 Gorham, L. W. & Waymouth, C. (1965) Proc. SOC. Exp. Biol. Med. 119,287-290 Hascall, V. C. & Sajdera, S . W. (1969)J. Biol. Chem. 244,2384-2396 Jimenez, S . A., Dehm, P. & Prockop, D. J. (1971) FEBSLett. 17,245-249 Lowry, 0. H., Rosebrough, N. J., Farr, A. L. &Randall, R. J. (1951)J. Biol. Chem. 193,265-
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Immunochemical analysis of cartilage proteoglycans
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1. Proteoglycan was obtained from bovine nasal cartilage by a procedure involving sequential extraction with a low-ionic-strength KCl solution, then a high-ionic-strength CaCl2 solution. Purification was by CsCl-density-gradient centrifugation. 2. The CaCl2- extracted proteoglycan was subjected to proteolytic degradation by papain, trypsin, cathepsin D, cathepsin B, lysosomal elastase or cathep...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 3 1 شماره
صفحات -
تاریخ انتشار 1975